中国典型储粮生态区低温储粮的优化集成方案

根据储粮生态环境,将我国分为三个典型的低温储粮生态区域,即低温区、中温区和高温区。提出了三个储粮生态区域低温储粮对仓房隔热条件和气密条件的要求及不同地区低温储粮的优化集成方案。低温区最佳的低温储粮模式是:冬季通风降温 夏季隔热 适当控温;中温区最佳的低温储粮模式是:冬季机械通风降温 夏季严格隔热 必要的控温;高温区最佳的低温储粮模式为:冬季机械通风降温或谷物冷却机降温 夏季严格隔热 谷物冷却机降温。同时给出了三个储粮生态区域低温储粮优化方案的工艺流程图。     

Inheritance of male fertility restoration of the cytoplasmic-nuclear male-sterile line NJCMS1A of soybean [Glycine max (L) Merr.]

At present, no report on inheritance of male fertility restoration has been released, yet more than 10 cytoplasmic-nuclear male-sterile soybean lines as well as their maintainers and restorers have been developed. Based on our previous work, 25 restorers for the male-sterile line NJCMS1A were identified and the inheritance of male fertility restoration for these restorers was studied. The results showed that F₁s between NJCMS1A and its restorers were completely male-fertile. The numbers of fertile and sterile plants in the F₂ population of Cross I (NJCMS1A × N23601) and Cross II (NJCMS1A × N23683) corresponded to a segregation ratio of 15:1, and the numbers of non-segregation lines, 3:1 segregation lines and 15:1 segregation lines in F_2:3 of the same two crosses fitted a 7:4:4 genotypic segregation ratio. The testcross BC₁F₁s between the F₁s of the above two crosses and NJCMS1A, NJCMS1B showed a 3:1 segregation ratio. Accordingly, it was inferred that two pairs of duplicate dominant genes controlled the male fertility restoration of NJCMS1A in both crosses. Meanwhile, F₂ of other 23 crosses between NJCMS1A and its 23 restorers showed a fertility segregation ratio of 3:1 or 15:1. The F₁s of the five testcrosses between NJCMS1A and the F₁s of five crosses selected from the above 23 crosses showed that fertility segregation was 3:1 in BC₁F₁s between NJCMS1A and F₁s of the crosses of which fertility segregation fitted 15:1 in F₂ population, while fertility segregation in BC₁F₁s was 1:1 for those fertility segregation fitted 3:1 in F₂ population. Allelism tests showed that restore genes of all restorers in the experiment were allelic to two pairs of dominant genes. All results showed that some restorers bore one pair of dominant restore gene and the others bore two pairs of duplicate dominant gene. The mechanism of F₁ male sterility of the cross N8855 × N2899 was discussed.     

灵芝LZ-8在烟草中的表达及产物特性的初步研究

将从灵芝中克隆的LZ-8基因构建成原核表达质粒pET-30a-lz8，在大肠杆菌BL21中表达。SDS-PAGE表明其分子量约为14kD，与推算出的氨基酸分子量相似。经金属镍离子螯合柱纯化后的原核表达产物为单一条带，纯化产物免疫兔子获得了高效价的抗血清。将该基因亚克隆到植物双元表达载体中，用农杆菌介导的方法转化烟草。经ELISA实验定量测定，重组LZ-8达到了烟草可溶性蛋白的0．18％（每克新鲜叶片约含149．82μgLZ-8重组蛋白）。体外活性检测表明，重组蛋白能有效地提高VeroE6细胞中肿瘤坏死因子（TNF-α）基因的mRNA转录水平，具有免疫调节相关功能。     

Comparison of QTL for Grain Number per Panicle in Three Populations Sharing 3 Parents and Fine Mapping of Gnlc

Complex traits, such as yield components, are inherited in a quantitative manner and typically controlled by quantitative trait loci （QTL）. Grain number per panicle （GN） is an important component of yield in rice and has been studied for QTL mapping in our lab （Yu et al., 1997; Xing et al., 2002）. Further discovery of QTL for GN and fine mapping will provide rich of gene resources for high yield breeding by marker assistant selection. Gene cloning is helpful to understand the biological mechanism underlying GN and instruct the application of gene engineering in rice yield breeding. In recent years, near-isogenic lines （NILs） for grain number have been reported for gene fine mapping （Tian et al., 2006; Zhang et al., 2006） and gene cloning （Ashikari et al., 2005）. However, so far, this kind of research is insufficiency for systematically elucidating the genetic bases and regulatory mechanism involved in GN. In this study, we compare the locations and genetic effects of QTL for GN detected in three sets of recombinant inbred line populations （RILs） sharing three parents, and fine map a new major QTL, Gnlc, commonly detected in the 3 populations.     

用现代林业发展理念推动和龙市林业产业建设

通过对和龙市林业局林业产业建设的情况分析,运用现代林业发展理念,提出推动和龙市林业产业建设的合理化建议,阐述今后十年和龙市林业局林业产业建设的发展方向和构想。希望打造一批区域优势明显、森林资源丰富、综合效益高、示范带动能力强的优势特色林业产业基地。     

Antifungal properties of Pseudarthria viscida

The extracts of leaf, root, stem, and the callus obtained from Pseudarthria viscida showed significant inhibitory activity against some fungal pathogens causing major diseases in crop plants and stored food grains.     

茶油产品品质等级标准制订说明

油茶是我国特有的食用油料树种。茶油富含人体必须的油酸，是优质的具有保健功能的食用植物油。为规范油茶生产和茶油等级，因而必须制定标准。本标准包括油茶产地生态环境条件、无公害油茶林生态系统管理和茶油质量等级3部分。     

落叶松根际pH值与供磷水平及土壤磷有效性的关系

植物在生长过程中由于根分泌物的作用 ,根际ｐＨ值将发生不同于土体的变化。这种变化在植物利用难溶性营养元素 (如磷素 )上是极其重要的。迄今为止关于草本植物和作物根际ｐＨ值变化与土壤中难溶性磷利用的研究已有很多报道。Ｇｒｉｎｓｔｅｄ和Ｈｅｄｌｅｙ( 1 986)研究发现生长 35天的油菜幼苗根际ｐＨ值可降低 2 4个单位 (由 6 5到 4 1 ) ,相应其根际有效磷的浓度增加 1 0倍 (王庆仁等 ,1 998)。Ｍａｒｃｈｎｅｒ( 1 986)发现白羽扇豆在缺磷土壤中形成排根 ,排根分泌的有机酸能降低根际土壤的ｐＨ值以促进其对磷的吸收。目前人们普遍认…     

林业植物新品种保护制度的实施与桉树良种化发展机遇的探讨

为了保护植物育种者的权利和利益,使育种者能从新品种的选育、生产和销售中收回投资和获得利润,鼓励继续投资和培育新品种,我国于1999年4月23日正式实施<中华人民共和国植物薪品种保护条例>,对植物新品种进行知识产权保护.本文综述了我国林业植物保护制度实施后的总体概况、特点,以及与国际植物新品种保护联盟(upov)近期的合作与交流.结合桉树在我国的发展利用前景,和近15a来全面系统的引种、改良和良种选育成果,认为桉树是我国南方最具林业产业化的人工林树种,也是林业最具植物新品种保护的树种之一.     

株洲市港水污染特征研究

根据株洲市1991～1997年5条主要河港的水质监测结果,对市区港水污染特征进行了分析.由于河港周围污染源的种类和数量不同、港水的污染程度和主要污染物种类也不尽相同.各港水综合污染指数(P)表明霞湾港污染最重,其次是铜塘港.再次为建宁港和白石港.枫溪港污染最轻.枫溪、建宁和白石港周围的工厂较少,但接纳了市区大部分生活污水.因此.有机污染物和富营养化物质是港水中的主要污染物;铜塘港和霞湾港中几乎全部为工业区的废水,因此,其污染物成分复杂,主要污染物为有机物和重金属离子.